Webb1 jan. 2024 · Ni-NTA-Nanogold®for His-tag labeling & detection. no silver/gold enhancement necessary! Ni-NTA-Nanogold ® is designed for detection or localization of polyhistidine (his) -tagged fusion proteins … WebbHuman AGT Protein, His Tag: 规格: 10ug, 20ug: 物种: Human: 宿主: HEK293 Cells: 纯度 >98%, determined by SDS-PAGE: 内毒素水平: 1.0 EU/ug determined by the LAL method: 缓冲体系: Lyophilized from sterile PBS, pH 7.4, and 0.01% Tween80 are added as protectants: 保存条件: Aliquot and store at -20℃ to -80℃. Avoid repeated ...
His Tag Antibodies Thermo Fisher Scientific - CN
WebbRecombinant Protein A/G, His-tag. >96% by SDS-PAGE and RP-HPLC analyses. The recombinant Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 3 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein … WebbHis-tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. Our His-tag antibodies also come with the option of being HRP, biotin, or fluorophore conjugated to meet all your research needs. clerk connect lafourche
常用蛋白标签对比 - 知乎
WebbHis标签是指六个组氨酸残基组成的融合标签，可插入在目的蛋白的C末端或N末端。. 当某一个标签的使用，一是能构成表位利于检测；二是构成独特的结构特征（结合配体）利 … Webb28 nov. 2024 · 1、纯化标签在N端，纯化时所有表达出纯化标签的都可以挂在纯化柱上。 纯化标签在C端，纯化时只有目的蛋白表达完全又能表达出纯化标签的才可以挂在纯化柱上。 蛋白表达中途截止的就没有纯化标签也就挂不到柱子上。 这也是N端比C端纯化出来的蛋白量高的原因。 2、对于比较难表达和蛋白太小容易被降解的蛋白，可以加在N端，可以 … WebbHIS-Tag蛋白纯化的首选标签. HIS-Tag本身的特性对目的蛋白没有影响，不会形成二聚体；. 分子量较小，只有0.84KD，对蛋白的下游应用不会产生影响；. 免疫原性低，可将纯化的蛋白直接注射动物进行免疫并制备抗体；. HIS-Tag与细菌的转录翻译机制兼容，有利于蛋白 ... bluff drama club